Hotspot

matK-trnK-UUU

LSC · 3411-4147 · 737 bp

Back to Astrocaryum

Pi 0.000798

MarkerSeek score 49.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 5
pi: 0.000798
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TACTTGAACCAGAGGCAGGA	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	815-913 bp	1.0	79.8
matK-trnK-UUU_p2	TACTTGAACCAGAGGCAGGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	814-912 bp	1.0	79.8
matK-trnK-UUU_p3	GAGGCAGGACCCATTTCATT	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	804-902 bp	1.0	78.8
matK-trnK-UUU_p4	GAGGCAGGACCCATTTCATT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	803-901 bp	1.0	78.8
matK-trnK-UUU_p5	TACTTGAACCAGAGGCAGGA	GTCGTGCAGTGCAATTCAAT	50.0	45.0	58.0	57.7	895-993 bp	1.0	78.4