Hotspot

rpl16

LSC · 78271-79752 · 1482 bp

Back to Astrebla

Pi 0.00405

MarkerSeek score 61.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 6
pi: 0.00405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTCCAGAAGGAAAACCTGGG	AGAGCCTTACCAAAAATCCCA	55.0	42.9	57.8	57.2	1629-1630 bp	1.0	64.1
rpl16_p2	CTCCAGAAGGAAAACCTGGG	AGCCTTACCAAAAATCCCAGA	55.0	42.9	57.8	57.2	1627-1628 bp	1.0	64.1
rpl16_p3	CTCCAGAAGGAAAACCTGGG	AAGAGCCTTACCAAAAATCCCA	55.0	40.9	57.8	57.9	1630-1631 bp	1.0	61.7
rpl16_p4	CTCCAGAAGGAAAACCTGGG	AGCCTTACCAAAAATCCCAGAA	55.0	40.9	57.8	57.9	1627-1628 bp	1.0	61.7
rpl16_p5	CTCCAGAAGGAAAACCTGGG	GAGCCTTACCAAAAATCCCAG	55.0	47.6	57.8	56.8	1628-1629 bp	1.0	61.5