Hotspot

petA

LSC · 60216-61178 · 963 bp

Back to Astrebla

Pi 0.00415

MarkerSeek score 61.10

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 4
pi: 0.00415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AATTCCGGGATCCACGATTG	TTATTGTTCTCGCCTCTGCC	50.0	50.0	58.0	58.0	1094 bp	1.0	79.8
petA_p2	GAAATTCCGGGATCCACGAT	TTATTGTTCTCGCCTCTGCC	50.0	50.0	57.7	58.0	1096 bp	1.0	78.0
petA_p3	AATTCCGGGATCCACGATTG	TCTGCCTTGGATTCTCCTCT	50.0	50.0	58.0	57.7	1080 bp	1.0	77.9
petA_p4	GAAATTCCGGGATCCACGAT	TCTGCCTTGGATTCTCCTCT	50.0	50.0	57.7	57.7	1082 bp	1.0	76.2
petA_p5	AATTCCGGGATCCACGATTG	CTCTGCCTTGGATTCTCCTC	50.0	55.0	58.0	57.4	1081 bp	1.0	75.1