Hotspot

atpI

LSC · 31460-32203 · 744 bp

Back to Astrebla

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 GCTGAGTTGAGAAAGAGATGGT TGCATGAATTGGGCTAAGCT 45.5 45.0 58.1 57.9 869 bp 1.0 62.4
atpI_p2 TGCTGAGTTGAGAAAGAGATGG TGCATGAATTGGGCTAAGCT 45.5 45.0 58.1 57.9 870 bp 1.0 62.4
atpI_p3 ACTTCAAGTTGCTGAGTTGAGA TGCATGAATTGGGCTAAGCT 40.9 45.0 57.9 57.9 879 bp 1.0 61.8
atpI_p4 GCTGAGTTGAGAAAGAGATGGT CAACCATGCATGAATTGGGC 45.5 50.0 58.1 58.3 875 bp 1.0 60.9
atpI_p5 TGCTGAGTTGAGAAAGAGATGG CAACCATGCATGAATTGGGC 45.5 50.0 58.1 58.3 876 bp 1.0 60.8