Hotspot

ndhF-rpl32

SSC · 104328-105240 · 913 bp

Back to Astrebla

Pi 0.0011

MarkerSeek score 59.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 913
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAAGAAAAAGCCCCAACCCC	TGACATCGAAGTACGTTTTT	50.0	35.0	58.0	53.2	1031 bp	1.0	41.8
ndhF-rpl32_p2	TAAGAAAAAGCCCCAACCCC	TTGACATCGAAGTACGTTTT	50.0	35.0	58.0	53.2	1032 bp	1.0	41.8
ndhF-rpl32_p3	TAAGAAAAAGCCCCAACCCC	TTTGACATCGAAGTACGTTT	50.0	35.0	58.0	53.2	1033 bp	1.0	41.8
ndhF-rpl32_p4	TAAGAAAAAGCCCCAACCCC	TTTTGACATCGAAGTACGTT	50.0	35.0	58.0	53.2	1034 bp	1.0	41.8
ndhF-rpl32_p5	TAAGAAAAAGCCCCAACCCC	TTTTTGACATCGAAGTACGT	50.0	35.0	58.0	53.2	1035 bp	1.0	41.8