Hotspot

trnN-GUU-ycf1

Genome · 103464-104079 · 616 bp

Back to Astragalus

Pi 0.116

MarkerSeek score 72.90

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: Genome
length_bp: 616
variable_sites: 407
pi: 0.116
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GCCTAGTGGACCCTAAGCTA	CTGTCCCGTGATAAACCCAG	55.0	55.0	57.9	58.0	80-1911 bp	0.909091	87.5
trnN-GUU-ycf1_p2	GAGAGAGAAGCGCTTTCACA	CTGTCCCGTGATAAACCCAG	50.0	55.0	57.9	58.0	80-2146 bp	0.621212	76.1
trnN-GUU-ycf1_p3	TGCTCGTGGTAATTGTAGGC	CTGTCCCGTGATAAACCCAG	50.0	55.0	58.0	58.0	80-1790 bp	0.5	71.7
trnN-GUU-ycf1_p4	GTAAGGTAATCCGGGGGAGA	CTGTCCCGTGATAAACCCAG	55.0	55.0	57.9	58.0	80-1465 bp	0.151515	57.1
trnN-GUU-ycf1_p5	TAAGGTAATCCGGGGGAGAC	CTGTCCCGTGATAAACCCAG	55.0	55.0	57.9	58.0	80-1464 bp	0.151515	57.1