Hotspot

trnR-ACG-trnN-GUU

Genome · 102905-103391 · 487 bp

Back to Astragalus

Pi 0.0811

MarkerSeek score 84.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 487
variable_sites: 294
pi: 0.0811
unique_haplotype_count: 62
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	55.0	50.0	57.8	57.6	80-623 bp	0.969697	86.1
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	80-661 bp	0.909091	84.2
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GTTCCTCAGTAGCTCAGTGG	55.0	55.0	57.8	57.3	80-662 bp	0.909091	81.3
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CCTCAGTAGCTCAGTGGTAGA	55.0	52.4	57.8	58.0	80-659 bp	0.969697	81.0
trnR-ACG-trnN-GUU_p5	GGCCTGTAGCTCAGAGGATT	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	58.9	57.7	80-672 bp	0.909091	78.9