Hotspot

psbE-petL

Genome · 61892-63023 · 1132 bp

Back to Astragalus

Pi 0.0599

MarkerSeek score 85.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1132
variable_sites: 650
pi: 0.0599
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	CGAAATAACTGGTTATAGTGGGC	45.0	43.5	58.0	57.3	80-1320 bp	0.954545	60.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CGAAATAACTGGTTATAGTGGGC	50.0	43.5	57.9	57.3	80-1309 bp	0.954545	60.4
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	CGAAATAACTGGTTATAGTGGGC	50.0	43.5	57.8	57.3	80-1232 bp	0.954545	59.6
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	CGAAATAACTGGTTATAGTGGGC	55.0	43.5	58.2	57.3	80-1227 bp	0.954545	59.2
psbE-petL_p5	ACCAACCCGCAATGAATAGG	CGAAATAACTGGTTATAGTGGGC	50.0	43.5	58.2	57.3	80-1311 bp	0.954545	58.9