Hotspot

ycf1

IRb · 112029-113200 · 1172 bp

Back to Astilbe

Pi 0.0058

MarkerSeek score 36.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1172
variable_sites: 17
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	TCATATCCCCACGAATCGGA	55.0	50.0	58.0	58.0	2117-2126 bp	1.0	81.0
ycf1_p2	CCCGGAACTTCTTCGTAGTG	TCATATCCCCACGAATCGGA	55.0	50.0	58.0	58.0	2022-2031 bp	1.0	81.0
ycf1_p3	CCGGAACTTCTTCGTAGTGG	TCATATCCCCACGAATCGGA	55.0	50.0	58.0	58.0	2021-2030 bp	1.0	81.0
ycf1_p4	GAGCTACTGAGGAACAACGG	CAAATATGTAGGGGGTGGGC	55.0	55.0	58.0	58.0	1593-1602 bp	1.0	80.9
ycf1_p5	CCGGAACTTCTTCGTAGTGG	CAAATATGTAGGGGGTGGGC	55.0	55.0	58.0	58.0	1497-1506 bp	1.0	80.9