Hotspot

ycf4-cemA

LSC · 63353-64295 · 943 bp

Back to Astilbe

Pi 0.00848

MarkerSeek score 56.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 19
pi: 0.00848
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	826-1077 bp	1.0	83.6
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	AAGAGAGAGATCCACCAGGG	45.0	55.0	57.8	57.8	832-1083 bp	1.0	82.9
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	TCGTAACCCAGGATTCCAGA	45.0	50.0	57.8	57.8	864-1115 bp	1.0	82.3
ycf4-cemA_p4	GCCATTCCCTTGACTCGTAC	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.3	58.1	876-1127 bp	1.0	82.0
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	CGTAACCCAGGATTCCAGAC	45.0	55.0	57.8	57.7	863-1114 bp	1.0	81.7