Hotspot

trnT-UGU-trnL-UAA

LSC · 48776-49795 · 1020 bp

Back to Astilbe

Pi 0.00961
MarkerSeek score 49.50
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1020
variable_sites
23
pi
0.00961
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AGTTGACGCCATACCCAATC GCCTTTTTGGGGATAGAGGG 50.0 55.0 58.0 57.9 2238-2347 bp 1.0 84.2
trnT-UGU-trnL-UAA_p2 AGTTGACGCCATACCCAATC AAGGATTCCTTTACCGACGC 50.0 50.0 58.0 58.0 1993-2035 bp 1.0 83.0
trnT-UGU-trnL-UAA_p3 AGTTGACGCCATACCCAATC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1776-1819 bp 1.0 82.9
trnT-UGU-trnL-UAA_p4 CTCCAGGAATAGTTGACGCC AAGGATTCCTTTACCGACGC 55.0 50.0 58.1 58.0 2003-2045 bp 1.0 82.8
trnT-UGU-trnL-UAA_p5 GGCTCCAGGAATAGTTGACG AAGGATTCCTTTACCGACGC 55.0 50.0 58.1 58.0 2005-2047 bp 1.0 82.8