Hotspot

psbZ-trnG-UCC

LSC · 37942-38574 · 633 bp

Back to Astilbe

Pi 0.0209

MarkerSeek score 68.20

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 31
pi: 0.0209
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	781-796 bp	1.0	82.5
psbZ-trnG-UCC_p2	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	782-797 bp	1.0	81.2
psbZ-trnG-UCC_p3	GTATTTGCTTCTCCTGACGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	784-799 bp	1.0	77.6
psbZ-trnG-UCC_p4	TGTATTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	785-800 bp	1.0	77.5
psbZ-trnG-UCC_p5	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	790-805 bp	1.0	76.1