Hotspot

psbC-trnS-UGA

LSC · 37079-37311 · 233 bp

Back to Astilbe

Pi 0.0215

MarkerSeek score 60.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 9
pi: 0.0215
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	353-359 bp	1.0	81.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.2	58.1	345-351 bp	1.0	80.8
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.2	58.1	346-352 bp	1.0	80.8
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	338-344 bp	1.0	80.8
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	433-439 bp	1.0	80.3