Hotspot

trnY-GUA-trnE-UUC

LSC · 32254-32317 · 64 bp

Back to Astilbe

Pi 0.025
MarkerSeek score 55.90
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
64
variable_sites
3
pi
0.025
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA CATAGTATGATGGCGGTCGG 50.0 55.0 57.8 58.0 224-229 bp 1.0 80.9
trnY-GUA-trnE-UUC_p2 ACCAGCGTAGACATATTGCC CATAGTATGATGGCGGTCGG 50.0 55.0 57.8 58.0 225-230 bp 1.0 80.8
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC CATAGTATGATGGCGGTCGG 55.0 55.0 58.4 58.0 203-208 bp 1.0 80.3
trnY-GUA-trnE-UUC_p4 ACGAATTTACAGTCCGTCCC CATAGTATGATGGCGGTCGG 50.0 55.0 57.6 58.0 204-209 bp 1.0 80.0
trnY-GUA-trnE-UUC_p5 CCAGCGTAGACATATTGCCA CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.8 58.3 168-173 bp 1.0 79.7