Hotspot

trnD-GUC-trnY-GUA

LSC · 31770-32169 · 400 bp

Back to Astilbe

Pi 0.00967

MarkerSeek score 59.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 9
pi: 0.00967
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	447-472 bp	1.0	79.8
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	446-471 bp	1.0	79.8
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	468-493 bp	1.0	78.6
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	467-492 bp	1.0	78.5
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	ATTTTTCCTTTCCGGGGTCG	50.0	50.0	58.5	58.5	522-530 bp	0.8	65.7