Hotspot

trnC-GCA-petN

LSC · 28893-29626 · 734 bp

Back to Astilbe

Pi 0.0079

MarkerSeek score 57.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 13
pi: 0.0079
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	824-849 bp	1.0	82.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	768-793 bp	1.0	82.3
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	885-910 bp	1.0	80.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	829-854 bp	1.0	80.4
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	786-811 bp	1.0	80.0