Hotspot

trnR-UCU-atpA

LSC · 10620-10829 · 210 bp

Back to Astilbe

Pi 0.0119

MarkerSeek score 61.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 210
variable_sites: 6
pi: 0.0119
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAAAGGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.5	58.1	364-375 bp	1.0	79.0
trnR-UCU-atpA_p2	ATGAAAGGCGTCCATTGTCT	CATTCACCGAGGAAGCAGAA	45.0	50.0	57.5	57.9	372-383 bp	1.0	78.8
trnR-UCU-atpA_p3	ATGAAAGGCGTCCATTGTCT	AAGACATTCACCGAGGAAGC	45.0	50.0	57.5	57.9	376-387 bp	1.0	78.8
trnR-UCU-atpA_p4	GGAATGAAAGGCGTCCATTG	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	57.4	58.1	367-378 bp	1.0	78.4
trnR-UCU-atpA_p5	GGAATGAAAGGCGTCCATTG	CATTCACCGAGGAAGCAGAA	50.0	50.0	57.4	57.9	375-386 bp	1.0	78.2