Hotspot

ycf1

SSC · 127254-132545 · 5292 bp

Back to Asteropyrum

Pi 0.0136

MarkerSeek score 60.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5292
variable_sites: 72
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TATAGCCTCCGGAATCAGCA	ACAAGTAAACGAAGCCCGAA	50.0	45.0	58.0	57.8	943 bp	1.0	80.1
ycf1_p2	ACCACAGTTCCTTGCATTGT	ACAAGTAAACGAAGCCCGAA	45.0	45.0	57.9	57.8	1318-1324 bp	1.0	79.7
ycf1_p3	ACCCGTGAAATAGCTAGGGT	ACAAGTAAACGAAGCCCGAA	50.0	45.0	58.1	57.8	1053 bp	1.0	79.1
ycf1_p4	ATGTCGTGCAATCGGTTCAT	ACAAGTAAACGAAGCCCGAA	45.0	45.0	58.3	57.8	1141-1147 bp	1.0	78.3
ycf1_p5	CACCCGTGAAATAGCTAGGG	ACAAGTAAACGAAGCCCGAA	55.0	45.0	57.8	57.8	1054 bp	1.0	78.2