Pi
0.0153
MarkerSeek score
55.10
Primer available
yes
Label
ndhA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhA_p1 | TGAATCTAGAATATCCTGTGCCT | TGGGAGAAGTTGATCGTTGA | 39.1 | 45.0 | 56.6 | 56.5 | 2158-2161 bp | 1.0 | 42.1 |
| ndhA_p2 | TGAATCTAGAATATCCTGTGCCT | TGGGAGAAGTTGATCGTTGAA | 39.1 | 42.9 | 56.6 | 57.2 | 2158-2161 bp | 1.0 | 42.1 |
| ndhA_p3 | TGAATCTAGAATATCCTGTGCC | TGGGAGAAGTTGATCGTTGA | 40.9 | 45.0 | 55.1 | 56.5 | 2158-2161 bp | 1.0 | 42.1 |
| ndhA_p4 | TGAATCTAGAATATCCTGTGCCT | ATGGGAGAAGTTGATCGTTGA | 39.1 | 42.9 | 56.6 | 57.0 | 2159-2162 bp | 1.0 | 42.1 |
| ndhA_p5 | ATGAATCTAGAATATCCTGTGCCT | TGGGAGAAGTTGATCGTTGA | 37.5 | 45.0 | 57.1 | 56.5 | 2159-2162 bp | 1.0 | 42.1 |