Hotspot

rps3-rpl16

IRa · 159432-159606 · 175 bp

Back to Asteropyrum

Pi 0.0294

MarkerSeek score 72.10

Primer available yes

Label rps3-rpl16

Feature Metadata

label_name: rps3-rpl16
feature_type: igs
region: IRa
length_bp: 175
variable_sites: 5
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl16_p1	TTCCCCTACAAACCATTCGC	TTCATACTAGGGGACGGTCC	50.0	55.0	58.2	57.9	358-363 bp	1.0	80.2
rps3-rpl16_p2	CCTACAAACCATTCGCGCTA	TTCATACTAGGGGACGGTCC	50.0	55.0	58.4	57.9	354-359 bp	1.0	78.8
rps3-rpl16_p3	TTCCCCTACAAACCATTCGC	AAACCAACGAGTCACACACT	50.0	45.0	58.2	57.6	303-308 bp	1.0	78.1
rps3-rpl16_p4	GGGTTCCCCTACAAACCATT	TTCATACTAGGGGACGGTCC	50.0	55.0	57.4	57.9	361-366 bp	1.0	76.7
rps3-rpl16_p5	CCTACAAACCATTCGCGCTA	AAACCAACGAGTCACACACT	50.0	45.0	58.4	57.6	299-304 bp	1.0	76.7