Hotspot

ndhE-ndhG

SSC · 121364-121548 · 185 bp

Back to Asteropyrum

Pi 0.0465

MarkerSeek score 76.40

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 185
variable_sites: 8
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGACACATAAGCGCTCGAA	CATTGCTATGGCTCGTCAGT	45.0	50.0	57.6	58.1	299-312 bp	1.0	82.2
ndhE-ndhG_p2	AGACACATAAGCGCTCGAAC	CATTGCTATGGCTCGTCAGT	50.0	50.0	58.4	58.1	298-311 bp	1.0	82.1
ndhE-ndhG_p3	CAAGACACATAAGCGCTCGA	CATTGCTATGGCTCGTCAGT	50.0	50.0	58.4	58.1	300-313 bp	1.0	82.1
ndhE-ndhG_p4	TCAAGACACATAAGCGCTCG	CATTGCTATGGCTCGTCAGT	50.0	50.0	58.4	58.1	301-314 bp	1.0	82.1
ndhE-ndhG_p5	AAGACACATAAGCGCTCGAA	CCTTGATAGGTGCCATTGCT	45.0	50.0	57.6	57.9	312-325 bp	1.0	81.9