Hotspot

ccsA-ndhD

SSC · 118721-118926 · 206 bp

Back to Asteropyrum

Pi 0.0464

MarkerSeek score 69.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 206
variable_sites: 9
pi: 0.0464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTATGCAGCTCTTCTGTGT	GGACCGCGAGAACTATTTGT	50.0	50.0	58.0	58.0	865-889 bp	1.0	83.8
ccsA-ndhD_p2	GCTATGCAGCTCTTCTGTGT	TTGCATTGCCTGGAATGAGT	50.0	45.0	58.0	58.1	1080-1104 bp	1.0	82.5
ccsA-ndhD_p3	GCTATGCAGCTCTTCTGTGT	CTTCAACATCTCCCGGTCAG	50.0	55.0	58.0	58.0	1362-1386 bp	1.0	82.3
ccsA-ndhD_p4	GCTATGCAGCTCTTCTGTGT	ACGAAATGGGAGGAGTAGCT	50.0	50.0	58.0	58.1	1152-1176 bp	1.0	81.9
ccsA-ndhD_p5	GCTATGCAGCTCTTCTGTGT	TGGACCGCGAGAACTATTTG	50.0	50.0	58.0	58.0	890 bp	0.5	59.8