Hotspot

rpl16-rps3

IRb · 86993-87167 · 175 bp

Back to Asteropyrum

Pi 0.0294

MarkerSeek score 72.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: IRb
length_bp: 175
variable_sites: 5
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TTCATACTAGGGGACGGTCC	TTCCCCTACAAACCATTCGC	55.0	50.0	57.9	58.2	358-363 bp	1.0	80.2
rpl16-rps3_p2	TTCATACTAGGGGACGGTCC	CCTACAAACCATTCGCGCTA	55.0	50.0	57.9	58.4	354-359 bp	1.0	78.8
rpl16-rps3_p3	AAACCAACGAGTCACACACT	TTCCCCTACAAACCATTCGC	45.0	50.0	57.6	58.2	303-308 bp	1.0	78.1
rpl16-rps3_p4	TTCATACTAGGGGACGGTCC	GGGTTCCCCTACAAACCATT	55.0	50.0	57.9	57.4	361-366 bp	1.0	76.7
rpl16-rps3_p5	AAACCAACGAGTCACACACT	CCTACAAACCATTCGCGCTA	45.0	50.0	57.6	58.4	299-304 bp	1.0	76.7