Hotspot

ycf4-cemA

LSC · 65010-65764 · 755 bp

Back to Asteropyrum

Pi 0.0195

MarkerSeek score 67.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 13
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTCCCTTGACTCGTACTG	GAAAGAAATCCACCAGGGCA	55.0	50.0	58.0	58.1	912-933 bp	1.0	84.3
ycf4-cemA_p2	AATTGGCCTACTTTTTGCGC	GAAAGAAATCCACCAGGGCA	45.0	50.0	57.9	58.1	850-871 bp	1.0	84.0
ycf4-cemA_p3	TGCGGAATTGGCCTACTTTT	GAAAGAAATCCACCAGGGCA	45.0	50.0	58.1	58.1	855-876 bp	1.0	83.9
ycf4-cemA_p4	CGTTCCCTTGACTCGTACTG	AGAAAGAAATCCACCAGGGC	55.0	50.0	58.0	57.8	913-934 bp	1.0	83.2
ycf4-cemA_p5	AATTGGCCTACTTTTTGCGC	AGAAAGAAATCCACCAGGGC	45.0	50.0	57.9	57.8	851-872 bp	1.0	82.8