Hotspot

rbcL-accD

LSC · 61390-62212 · 823 bp

Back to Asteropyrum

Pi 0.0259

MarkerSeek score 60.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 21
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCAAATGGAGCCTTGAACT	TAGCCCACACCTGTGTTCTA	45.0	50.0	58.0	58.0	965-970 bp	1.0	82.7
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	TAGCCCACACCTGTGTTCTA	50.0	50.0	58.0	58.0	1113-1118 bp	1.0	82.4
rbcL-accD_p3	AATGAGGGACGTGATCTTGC	TAGCCCACACCTGTGTTCTA	50.0	50.0	58.0	58.0	1016-1021 bp	1.0	82.4
rbcL-accD_p4	AGAGAGATCACCTTGGGCTT	TAGCCCACACCTGTGTTCTA	50.0	50.0	58.0	58.0	1295-1300 bp	1.0	81.8
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	TAGCCCACACCTGTGTTCTA	50.0	50.0	58.0	58.0	1592-1597 bp	1.0	81.7