Hotspot

trnT-UGU-trnL-UAA

LSC · 49672-51240 · 1569 bp

Back to Asteropyrum

Pi 0.0281

MarkerSeek score 64.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1569
variable_sites: 43
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CTAACCTCTGAGCTAAGCGG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.5	58.0	1605-1611 bp	1.0	80.1
trnT-UGU-trnL-UAA_p2	CTAACCTCTGAGCTAAGCGG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.5	58.1	1613-1619 bp	1.0	79.6
trnT-UGU-trnL-UAA_p3	CTAACCTCTGAGCTAAGCGG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.5	57.8	1628-1634 bp	1.0	78.3
trnT-UGU-trnL-UAA_p4	CTAACCTCTGAGCTAAGCGG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.5	58.2	1619-1625 bp	1.0	78.2
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.5	57.7	1625-1631 bp	1.0	77.9