Hotspot

psaA-ycf3

LSC · 44839-45650 · 812 bp

Back to Asteropyrum

Pi 0.0181

MarkerSeek score 58.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 13
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGGTTTGAATAA	50.0	45.0	57.9	57.5	821-912 bp	1.0	80.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GATCACGAGGCGGTTTGAAT	50.0	50.0	57.9	58.6	823-914 bp	1.0	79.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCACGAGGCGGTTTGAATA	50.0	45.0	57.9	57.3	822-913 bp	1.0	78.8
psaA-ycf3_p4	TTGAGAAATGACCCGGTCTG	TCACGAGGCGGTTTGAATAA	50.0	45.0	57.5	57.5	841-932 bp	1.0	77.7
psaA-ycf3_p5	AACTTCTGATTCCGGCGAAC	TCACGAGGCGGTTTGAATAA	50.0	45.0	58.6	57.5	771-862 bp	1.0	77.1