Hotspot

rpoC1-rpoB

LSC · 24540-24565 · 26 bp

Back to Asteropyrum

Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	CCAACCATCTCTCCGTTAGG	TTTGGCTCTGGAACTGAACC	55.0	50.0	57.4	58.0	200-205 bp	1.0	75.4
rpoC1-rpoB_p2	CCCAACCATCTCTCCGTTAG	TTTGGCTCTGGAACTGAACC	55.0	50.0	57.4	58.0	201-206 bp	1.0	75.4
rpoC1-rpoB_p3	CCAACCATCTCTCCGTTAGG	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.4	58.0	223-228 bp	1.0	75.2
rpoC1-rpoB_p4	CCCAACCATCTCTCCGTTAG	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.4	58.0	224-229 bp	1.0	75.2
rpoC1-rpoB_p5	CCAACCATCTCTCCGTTAGG	ACGATCTTTGGCTCTGGAAC	55.0	50.0	57.4	57.9	206-211 bp	1.0	74.7