Hotspot

atpF-atpH

LSC · 13824-14329 · 506 bp

Back to Asteropyrum

Pi 0.0201

MarkerSeek score 70.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 10
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	601-619 bp	1.0	81.9
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.3	58.0	628-646 bp	1.0	80.0
atpF-atpH_p3	AGTGACCCAAGGAAACGAAA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.3	58.0	600-618 bp	1.0	76.4
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	GGTCGTGGCATTAGCACTTT	50.0	50.0	58.0	58.8	590-608 bp	1.0	75.6
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GTCGTGGCATTAGCACTTTT	50.0	45.0	58.0	57.0	589-607 bp	1.0	74.3