Hotspot

rps16-trnQ-UUG

LSC · 6627-7968 · 1342 bp

Back to Asteropyrum

Pi 0.017
MarkerSeek score 67.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1342
variable_sites
22
pi
0.017
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1407-1436 bp 1.0 82.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1386-1415 bp 1.0 82.1
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1397-1426 bp 1.0 81.6
rps16-trnQ-UUG_p4 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1409-1438 bp 1.0 80.5
rps16-trnQ-UUG_p5 CCACAACCGATCATGTCCTT TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.9 57.3 1411-1440 bp 1.0 77.5