Hotspot

ycf1

SSC · 123589-128682 · 5094 bp

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Pi 0.00786
MarkerSeek score 45.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5094
variable_sites
162
pi
0.00786
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTGGAGAATTAGGATAGGGAAGA AGAATTGAGACTTTAGAGACAGT 41.7 34.8 57.7 54.8 302 bp 0.916667 41.2
ycf1_p2 GGAGAATTAGGATAGGGAAGAAGG AGAATTGAGACTTTAGAGACAGT 45.8 34.8 57.6 54.8 299 bp 0.916667 41.2
ycf1_p3 CTGGAGAATTAGGATAGGGAAGA AGAATTGAGACTTTAGAGACAGT 43.5 34.8 56.3 54.8 301 bp 0.916667 41.2
ycf1_p4 TCTGTGCAATTTTCTATCGAAGT AGAATTGAGACTTTAGAGACAGT 34.8 34.8 56.5 54.8 245 bp 0.916667 40.9
ycf1_p5 TGTTTCTGTGCAATTTTCTATCGA AGAATTGAGACTTTAGAGACAGT 33.3 34.8 57.4 54.8 249 bp 0.916667 40.8