Hotspot

ndhE-ndhG

SSC · 117982-118209 · 228 bp

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Pi 0.0102
MarkerSeek score 61.70
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
228
variable_sites
10
pi
0.0102
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 CACATAAGCGCCCTAACCAT TAATCGGCGCAATTGCTGTA 50.0 45.0 58.0 58.3 343-363 bp 1.0 83.3
ndhE-ndhG_p2 AGCGCCCTAACCATATTTCG TAATCGGCGCAATTGCTGTA 50.0 45.0 58.1 58.3 337-357 bp 1.0 82.6
ndhE-ndhG_p3 GCGCCCTAACCATATTTCGA TAATCGGCGCAATTGCTGTA 50.0 45.0 58.1 58.3 336-356 bp 1.0 82.6
ndhE-ndhG_p4 CAAGACACATAAGCGCCCTA TAATCGGCGCAATTGCTGTA 50.0 45.0 57.7 58.3 348-368 bp 1.0 81.0
ndhE-ndhG_p5 AGACACATAAGCGCCCTAAC TAATCGGCGCAATTGCTGTA 50.0 45.0 57.7 58.3 346-366 bp 1.0 81.0