Hotspot

accD-psaI

LSC · 58838-59794 · 957 bp

Back to Aster

Pi 0.0361
MarkerSeek score 73.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
957
variable_sites
98
pi
0.0361
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.91
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AAAAGGCGTTCTGAGTGAGT GAGGGAAAATTAAGAGTTGTCA 45.0 36.4 57.4 54.0 852-1044 bp 1.0 52.0
accD-psaI_p2 AAAGGCGTTCTGAGTGAGTT GAGGGAAAATTAAGAGTTGTCA 45.0 36.4 57.4 54.0 851-1043 bp 1.0 52.0
accD-psaI_p3 AAAAGGCGTTCTGAGTGAGT AGGGAAAATTAAGAGTTGTCA 45.0 33.3 57.4 52.6 851-1043 bp 1.0 52.0
accD-psaI_p4 AAAGGCGTTCTGAGTGAGTT AGGGAAAATTAAGAGTTGTCA 45.0 33.3 57.4 52.6 850-1042 bp 1.0 52.0
accD-psaI_p5 AAAGGCGTTCTGAGTGAGTT GAGGGAAAATTAAGAGTTGTCAT 45.0 34.8 57.4 54.6 851-1043 bp 1.0 52.0