Hotspot

ycf3-trnS_gga

LSC · 44311-45180 · 870 bp

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Pi 0.00958
MarkerSeek score 65.00
Primer available yes
Label ycf3-trnS_gga

Feature Metadata

label_name
ycf3-trnS_gga
feature_type
igs
region
LSC
length_bp
870
variable_sites
30
pi
0.00958
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS_gga_p1 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1014-1045 bp 1.0 71.9
ycf3-trnS_gga_p2 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1015-1046 bp 1.0 71.9
ycf3-trnS_gga_p3 TTCTCCTGAAGTTGTCGGAA CCTCGGTAAGCAAAAGCCTA 45.0 50.0 56.4 57.6 1004-1035 bp 1.0 70.5
ycf3-trnS_gga_p4 TTTCTCCTGAAGTTGTCGGA CCTCGGTAAGCAAAAGCCTA 45.0 50.0 56.4 57.6 1005-1036 bp 1.0 70.5
ycf3-trnS_gga_p5 TTCTCCTGAAGTTGTCGGAA GATTCGAACCCTCGGTAAGC 45.0 55.0 56.4 58.4 1013-1044 bp 1.0 70.2