Hotspot

psaA-ycf3

LSC · 41616-42381 · 766 bp

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Pi 0.00569
MarkerSeek score 61.30
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
766
variable_sites
19
pi
0.00569
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GAGAAATGACCCGGTCTAGC AGCCGCTGAGTATTGGAAAC 55.0 50.0 57.8 58.3 947-1006 bp 1.0 80.1
psaA-ycf3_p2 GAGAAATGACCCGGTCTAGC CAAGCCGCTGAGTATTGGAA 55.0 50.0 57.8 58.3 949-1008 bp 1.0 80.1
psaA-ycf3_p3 GAGAAATGACCCGGTCTAGC AAGCCGCTGAGTATTGGAAA 55.0 45.0 57.8 57.5 948-1007 bp 1.0 78.3
psaA-ycf3_p4 TTTTACTTCTGGTTCCGGCG AGCCGCTGAGTATTGGAAAC 50.0 50.0 58.5 58.3 882-941 bp 1.0 78.3
psaA-ycf3_p5 TTTTACTTCTGGTTCCGGCG CAAGCCGCTGAGTATTGGAA 50.0 50.0 58.5 58.3 884-943 bp 1.0 78.3