Hotspot

trnG_gcc-trnfM_cau

LSC · 36259-36469 · 211 bp

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Pi 0.0165
MarkerSeek score 62.20
Primer available yes
Label trnG_gcc-trnfM_cau

Feature Metadata

label_name
trnG_gcc-trnfM_cau
feature_type
igs
region
LSC
length_bp
211
variable_sites
15
pi
0.0165
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG_gcc-trnfM_cau_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 265-294 bp 1.0 86.5
trnG_gcc-trnfM_cau_p2 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 301-330 bp 1.0 86.2
trnG_gcc-trnfM_cau_p3 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 259-288 bp 1.0 86.1
trnG_gcc-trnfM_cau_p4 TCTTTGCCAAGGAGAAGACG TTCAGTTCTTCTCTTCGGCG 50.0 50.0 57.8 57.9 323-352 bp 1.0 85.8
trnG_gcc-trnfM_cau_p5 TCTTTGCCAAGGAGAAGACG TCACGGGTTCAAATCCTGTC 50.0 50.0 57.8 57.8 258-287 bp 1.0 85.5