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psbZ-trnG_gcc

LSC · 35861-36187 · 327 bp

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Pi 0.00833
MarkerSeek score 60.40
Primer available yes
Label psbZ-trnG_gcc

Feature Metadata

label_name
psbZ-trnG_gcc
feature_type
igs
region
LSC
length_bp
327
variable_sites
9
pi
0.00833
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG_gcc_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 471-502 bp 1.0 81.3
psbZ-trnG_gcc_p2 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 469-500 bp 1.0 80.9
psbZ-trnG_gcc_p3 CTCCTGATGGTTGGTCAAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 465-496 bp 1.0 76.1
psbZ-trnG_gcc_p4 TGCTTCTCCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.9 57.8 470-501 bp 1.0 75.5
psbZ-trnG_gcc_p5 TCTCCTGATGGTTGGTCAAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.8 57.8 466-497 bp 1.0 73.7