Hotspot

trnG_ucc-trnT_ggu

LSC · 30868-31126 · 259 bp

Back to Aster

Pi 0.0714
MarkerSeek score 68.70
Primer available yes
Label trnG_ucc-trnT_ggu

Feature Metadata

label_name
trnG_ucc-trnT_ggu
feature_type
igs
region
LSC
length_bp
259
variable_sites
56
pi
0.0714
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.77
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG_ucc-trnT_ggu_p1 ATGAAACCGAAAGACCCCTT ACTGAGTTAAAAGGAAAGGGCT 45.0 40.9 57.0 57.5 359-525 bp 0.5 42.8
trnG_ucc-trnT_ggu_p2 AATGAAACCGAAAGACCCCT ACTGAGTTAAAAGGAAAGGGCT 45.0 40.9 57.0 57.5 360-526 bp 0.5 42.7
trnG_ucc-trnT_ggu_p3 AATGAAACCGAAAGACCCCT CACTGAGTTAAAAGGAAAGGGC 45.0 45.5 57.0 57.5 361-527 bp 0.5 42.3
trnG_ucc-trnT_ggu_p4 ATGAAACCGAAAGACCCCTT CACTGAGTTAAAAGGAAAGGGC 45.0 45.5 57.0 57.5 360-526 bp 0.5 42.3
trnG_ucc-trnT_ggu_p5 GAATGAAACCGAAAGACCCCT ACTGAGTTAAAAGGAAAGGGCT 47.6 40.9 58.2 57.5 361-527 bp 0.5 40.8