Hotspot

trnS_gcu-trnC_gca

LSC · 8519-9315 · 797 bp

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Pi 0.0116
MarkerSeek score 64.80
Primer available yes
Label trnS_gcu-trnC_gca

Feature Metadata

label_name
trnS_gcu-trnC_gca
feature_type
igs
region
LSC
length_bp
797
variable_sites
36
pi
0.0116
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS_gcu-trnC_gca_p1 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 928-957 bp 1.0 83.7
trnS_gcu-trnC_gca_p2 ACGGAAAGAGAGGGATTCGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 910-939 bp 1.0 81.6
trnS_gcu-trnC_gca_p3 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 864-893 bp 1.0 80.2
trnS_gcu-trnC_gca_p4 AACGGAAAGAGAGGGATTCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.3 58.1 929-958 bp 1.0 79.5
trnS_gcu-trnC_gca_p5 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 846-875 bp 1.0 78.2