Hotspot

rpl2-trnH

IRa · 157814-157856 · 43 bp

Back to Astelia

Pi 0.116

MarkerSeek score 74.40

Primer available yes

Label rpl2-trnH

Feature Metadata

label_name: rpl2-trnH
feature_type: igs
region: IRa
length_bp: 43
variable_sites: 5
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH_p1	TTCTTCGTCGCCGTAAATAG	TGGATCAAGGCAGTGGATTG	45.0	50.0	55.7	57.9	122 bp	1.0	64.0
rpl2-trnH_p2	TTCTTCGTCGCCGTAAATAG	GGATCAAGGCAGTGGATTGT	45.0	50.0	55.7	57.9	121 bp	1.0	64.0
rpl2-trnH_p3	TTCTTCGTCGCCGTAAATAG	GTGGATCAAGGCAGTGGATT	45.0	50.0	55.7	57.9	123 bp	1.0	63.9
rpl2-trnH_p4	TTCTTCGTCGCCGTAAATAG	CGGATGTAGCCAAGTGGATC	45.0	55.0	55.7	58.1	136 bp	1.0	63.7
rpl2-trnH_p5	TTCTTCGTCGCCGTAAATAG	GATTGTGAATCCACCATGCG	45.0	50.0	55.7	57.5	107 bp	1.0	61.7