Hotspot

psaC-ndhE

SSC · 120436-120768 · 333 bp

Back to Astelia

Pi 0.037

MarkerSeek score 64.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 333
variable_sites: 12
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CAGGCTCGTACACATTGAGT	ACTCGTATCAATCAATCGAATTTGT	50.0	32.0	57.6	57.5	431 bp	1.0	43.9
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	ACTCGTATCAATCAATCGAATTTGT	55.0	32.0	58.3	57.5	447 bp	1.0	43.8
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	ACTCGTATCAATCAATCGAATTTGT	55.0	32.0	58.3	57.5	448 bp	1.0	43.8
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	ACTCGTATCAATCAATCGAATTTGT	50.0	32.0	58.1	57.5	471 bp	1.0	43.6
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	ACTCGTATCAATCAATCGAATTTGT	50.0	32.0	57.6	57.5	477 bp	1.0	43.5