Hotspot

ndhF-rpl32

SSC · 115512-116476 · 965 bp

Back to Astelia

Pi 0.0271

MarkerSeek score 65.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 965
variable_sites: 25
pi: 0.0271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAATTGGTCGTGTGAAC	TTGAATGTCCGGTGGAAACC	50.0	50.0	58.0	58.4	1569-1609 bp	1.0	80.4
ndhF-rpl32_p2	TGTTGCTGTCGGAATAAGCA	TTGAATGTCCGGTGGAAACC	45.0	50.0	57.8	58.4	1154-1194 bp	1.0	80.4
ndhF-rpl32_p3	GGTCGTGTGAACCAAAAACC	TTGAATGTCCGGTGGAAACC	50.0	50.0	57.8	58.4	1561-1601 bp	1.0	79.2
ndhF-rpl32_p4	AGATTTAGCAACTGCACCGA	TTGAATGTCCGGTGGAAACC	45.0	50.0	57.5	58.4	1778-1818 bp	1.0	76.6
ndhF-rpl32_p5	CTGCAATTGGTCGTGTGAAC	CGTCGAGATTCCAGGATTCA	50.0	50.0	58.0	57.1	2188-2215 bp	1.0	75.9