Hotspot

rps3-rps19

LSC · 85955-86444 · 490 bp

Back to Astelia

Pi 0.0168

MarkerSeek score 69.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 8
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TGTAGACCCGCGGAAAAATT	TCTGACTTTCGCGAGACATG	45.0	50.0	57.8	58.0	640-650 bp	1.0	81.2
rps3-rps19_p2	TTGTAGACCCGCGGAAAAAT	TCTGACTTTCGCGAGACATG	45.0	50.0	57.8	58.0	641-651 bp	1.0	81.2
rps3-rps19_p3	TGTAGACCCGCGGAAAAATT	TTGCGCCTACTCTGACTTTC	45.0	50.0	57.8	57.9	650-660 bp	1.0	80.7
rps3-rps19_p4	TTGTAGACCCGCGGAAAAAT	TTGCGCCTACTCTGACTTTC	45.0	50.0	57.8	57.9	651-661 bp	1.0	80.7
rps3-rps19_p5	TGTAGACCCGCGGAAAAATT	CCTACTCTGACTTTCGCGAG	45.0	55.0	57.8	57.8	645-655 bp	1.0	79.9