Hotspot

psbT-psbN

LSC · 76780-76838 · 59 bp

Back to Astelia

Pi 0.0678

MarkerSeek score 70.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 4
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	128 bp	1.0	81.6
psbT-psbN_p2	TTTTCGAGAACCGCCTAAGG	TTCGAAGAACACGGGGACTA	50.0	50.0	57.9	58.4	123 bp	1.0	78.8
psbT-psbN_p3	CGCCTAAGGTTCCGACTAAA	ATCCATTCGAAGAACACGGG	50.0	50.0	57.3	58.0	117 bp	1.0	77.3
psbT-psbN_p4	TTTTCGAGAACCGCCTAAGG	TCGAAGAACACGGGGACTAG	50.0	55.0	57.9	58.8	122 bp	1.0	75.3
psbT-psbN_p5	TTTTCGAGAACCGCCTAAGG	CATTCGAAGAACACGGGGAC	50.0	55.0	57.9	58.9	125 bp	1.0	74.5