Hotspot

ndhJ-ndhK

LSC · 51085-51165 · 81 bp

Back to Astelia

Pi 0.0247

MarkerSeek score 66.70

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 2
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GCAATCGAAGCCCAAAGATC	CAGACAGAATTCTTTTGTGCAGA	50.0	39.1	57.5	57.6	177 bp	1.0	49.6
ndhJ-ndhK_p2	GCAATCGAAGCCCAAAGATC	TCAGACAGAATTCTTTTGTGCAG	50.0	39.1	57.5	57.6	178 bp	1.0	49.6
ndhJ-ndhK_p3	GCAATCGAAGCCCAAAGATC	TCAGACAGAATTCTTTTGTGCA	50.0	36.4	57.5	56.5	178 bp	1.0	48.9
ndhJ-ndhK_p4	GTCCCAGTCCTCGGTTTTTA	CAGACAGAATTCTTTTGTGCAGA	50.0	39.1	57.2	57.6	219 bp	1.0	46.5
ndhJ-ndhK_p5	GTCCCAGTCCTCGGTTTTTA	TCAGACAGAATTCTTTTGTGCAG	50.0	39.1	57.2	57.6	220 bp	1.0	46.5