Hotspot

psbC-trnS

LSC · 36815-36951 · 137 bp

Back to Astelia

Pi 0.073

MarkerSeek score 82.50

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 10
pi: 0.073
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	TCGATCGTGATTTGGAACCC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	276 bp	1.0	82.6
psbC-trnS_p2	TCGATCGTGATTTGGAACCC	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	269 bp	1.0	82.4
psbC-trnS_p3	AACCCGTTCTTTCCATGACC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	261 bp	1.0	82.1
psbC-trnS_p4	TGGAACCCGTTCTTTCCATG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	264 bp	1.0	82.1
psbC-trnS_p5	TCGATCGTGATTTGGAACCC	AGCTCTGGTCTTGAAAACCG	50.0	50.0	58.0	58.1	254 bp	1.0	82.1