Hotspot

trnD-trnY

LSC · 31960-32253 · 294 bp

Back to Astelia

Pi 0.0386

MarkerSeek score 65.30

Primer available yes

Label trnD-trnY

Feature Metadata

label_name: trnD-trnY
feature_type: igs
region: LSC
length_bp: 294
variable_sites: 11
pi: 0.0386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-trnY_p1	GTGCTCTGACCGATTGAACT	CTGGTTCAAATCCAGCTCGG	50.0	55.0	57.9	58.9	337-346 bp	1.0	76.7
trnD-trnY_p2	GGATCCTAGTTCGGGACTGA	CTGGTTCAAATCCAGCTCGG	55.0	55.0	57.9	58.9	394-403 bp	1.0	76.2
trnD-trnY_p3	GTGCTCTGACCGATTGAACT	TCTACGCTGGTTCAAATCCA	50.0	45.0	57.9	56.9	343-352 bp	1.0	74.8
trnD-trnY_p4	GGATCCTAGTTCGGGACTGA	TCTACGCTGGTTCAAATCCA	55.0	45.0	57.9	56.9	400-409 bp	1.0	74.3
trnD-trnY_p5	ATCGGATCCTAGTTCGGGAC	CTGGTTCAAATCCAGCTCGG	55.0	55.0	58.4	58.9	397-406 bp	1.0	73.7