Hotspot

trnS-trnG

LSC · 8861-9717 · 857 bp

Back to Astelia

Pi 0.0264

MarkerSeek score 69.60

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 857
variable_sites: 22
pi: 0.0264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	940-963 bp	1.0	68.8
trnS-trnG_p2	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	941-964 bp	1.0	68.8
trnS-trnG_p3	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	994-1017 bp	1.0	68.1
trnS-trnG_p4	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	932-955 bp	1.0	67.6
trnS-trnG_p5	AATCTGCCGCTTTAGTCCAC	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.3	57.1	941-964 bp	1.0	66.9