Hotspot

rps16-trnQ

LSC · 5840-7518 · 1679 bp

Back to Astelia

Pi 0.0181

MarkerSeek score 65.90

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1679
variable_sites: 30
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1770-1777 bp	1.0	81.4
rps16-trnQ_p2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1768-1775 bp	1.0	81.4
rps16-trnQ_p3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1776-1783 bp	1.0	81.3
rps16-trnQ_p4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1777-1784 bp	1.0	81.3
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1752-1759 bp	1.0	80.5