Hotspot

rps16

Genome · 3546-4624 · 1079 bp

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Pi 0.0978

MarkerSeek score 85.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1079
variable_sites: 305
pi: 0.0978
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCTGGCCCCATTAGATTCTC	AAGACCATACCGAGGTTGGA	55.0	50.0	58.1	58.1	1702-1721 bp	0.666667	77.2
rps16_p2	GCTGGCCCCATTAGATTCTC	CGAAGACCATACCGAGGTTG	55.0	55.0	58.1	58.1	1704-1723 bp	0.666667	77.0
rps16_p3	GCTGGCCCCATTAGATTCTC	ACAACAGCTCGTATCGAACC	55.0	50.0	58.1	58.0	1679-1698 bp	0.25	56.4
rps16_p4	GTTGTAAGCTGGCCCCATTA	ACAACAGCTCGTATCGAACC	50.0	50.0	57.9	58.0	1686-1705 bp	0.25	56.1
rps16_p5	CTCCGGATCATGCCTAACAG	ACAACAGCTCGTATCGAACC	55.0	50.0	57.8	58.0	1875 bp	0.083333	42.0